refractometer calibration procedure
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Press the ON/OFF key, then release. Clean any equipment contaminated with blood e.g. Refractometer and Polarimater Calibration From Bellingham + Stanley AG Fluids They are ideal for calibrating refractometers that have temperature control in both food and industrial applications as well as instruments that have AG Fluid temperature compensation, such as the OPTi digital hand held refractometers and RFM700 Series refractometers. Water is used first to calibrate the instrument. Inspect the refractometer prism for scratches, chips, separations or other aberrations that may interfere with proper readings (Fig. Read the index of refraction using the telescope scale. Whether you’re looking for UKAS certified AG fluid, sucrose solution, replacement refractometers flaps, optical refractometer contact liquid or an inexpensive carry case for your digital refractometer, this is the area for you. Make sure the prism and sample well are completely dry. The average value of all your samples is the final wort correction factor. https://www.nist.gov/pml/weights-and-measures/laboratory-metrology/calibration-procedures. read more This creates a range of incidence angles, some of which (those less than the critical angle) will be completely reflected. These customers know that 0.0002 Urine SG accuracy must be validated regularly and Rudolph’s J57 refractometer ensures that the refractive index performance and calibration … checks, calibration verification, use, and preventive maintenance of 20 types of commonly used general laboratory equipment and follows the overall equipment management guidance provided in CLSI document QMS13. stream
1. LIQUID REFRACTOMETER STANDARDS with UKAS Certificates of Calibration (UKAS IS the United Kingdom Accreditation Authority) General purpose standards with shelf life of 12 months. 2. Press the ON/OFF key, then release. 1. Regardless of how many times the refractometer has been calibrated, it can always be restored to the initial factory calibration if required. Calibration is becoming an increasingly important part of quality assurance in terms of meeting standards and regulations. xÍ][SÛÈ~§ÿ0/[eo«. The Good Laboratory Practices (GLPs), Good Measurement Practices (GMPs), and Standard Operating Procedures (SOPs) that have not been updated are available on the linked pages as scanned PDF copies and are dated 1986. Turn on the light source. NBS Handbook 145, 1986, Handbook for the Quality Assurance of Metrological Measurements, by John K. Taylor, and Henry V. Oppermann is out of print and the majority of content has been updated and published in the publications noted below. Refractometer Calibration. The instrument should be checked by determining the Refractive Index of distilled water at 25 o C. If the refractive index of distilled water at 25 o C is not between 1.3320 – 1.3330, adjust the small square screw and at the back of telescope with the key provided with instrument. Calibration. Available in 30 ml sealed bottles with Brix values from 5%Brix to 60%Brix (approximately 1.34 to 1.44 RI), accuracy from 0.02%Brix to 0.1%Brix, depending on value. Share sensitive information only on official, secure websites. 1. 1. 3. The MA887 refractometer is an optical device that is simple and quick to use. Fill the well completely. A refractometer consists of a light source, filtered to a single wavelength, which is directed towards the prism-sample interface by a converging lens. 5.1 Operate the instrument as per S.O.P. Secure .gov websites use HTTPS The J357 offers 1 point, 2 point or a full multi-point calibration. Calibration can be done at 20°C, 25°C or any user selectable temperature within the available range. Note the temperature at the time of readings. Section 10, Glossary (Out of date; use the International Vocabulary of Metrology, Section 11, Bibliography (Legacy Handbook 145). Bring the reading setting to 1.3325 … 3.0 RESPONSIBILITY 3.1 Doing: Technical Assistant 3.2 Checking: Executive /Manager 4.0 ACCOUNTABILITY Head of the Department 5.0 PROCEDURE Frequency: Once in three months. …The digital refractometer eliminates the uncertainty associated with mechanical refractometers and is easily portable for ship, shore or home use. However, the organization of the sections and procedures on this and linked pages follows the format of the original Handbook 145, Table of Contents. Now you are ready to sample your wort. The J-Series Refractometer is chosen by more toxicology labs than any other brand. Most ATC refractome- Place a drop of urine in the designated spot on top of the refractometer. There are many updated GMPs, GLPs, and SOPs in the following publications that were originally published in HB 145; only updated versions are provided here when available. {RÔ1`oC°7©S}X+¾°² Å¿?Ó-ÁfñL¡e¬¯{._÷LOÏìµòbx^ìÝ»½VQ¤7Ùý¹×Þþµ×¸ÍöÎÒëá$-ÓÉ^ïî¢K²tåûûìàèý½½Å?³ÐþÉòl{ë˯l²½uÐßÞÚ;Lð Yÿj{KØç8,²jõÇö÷½]ÏìûØ5þWÿ½ßÞú³Ñ+ÒÉ ÍÍÝ°ÁNo³. To find the correct part number to order calibration for your MISCO refractometer: 1.) Alternatively, the Performance line is ideal for quality control on a … Prior to calibration, it is extremely important to completely clean the prism surfaces so no sample residue is present. Clean the refractometer by rinsing it with water and drying it with a clean paper towel … If dusty, rinse with water and wipe with a clean, soft, lint-free cloth. There are also quite a number of new GLPs, GMPs, and SOPs that were not originally part of Handbook 145. Zebra Refractometer Manual Correcting for Temperature If, during concentration measurement, the temperature is above or below 10°F to that of the temperature during calibration, it is recommended to recalibrate the unit at the testing temperature. Refractometer calibration and accessories At Refractometer Shop we have a range of calibration materials and accessories to help keep your refractometer in tip-top shape. CALIBRATION PROCEDURE Calibration should be performed daily, before measurements are made, when the battery has been replaced, or between a long series of measurements. 7. endobj
2.0 SCOPE This SOP is applicable for operation and calibration of Refractometer. Operate the instrument as mention in the operation procedure. Check the calibration at different temperatures of the refractometer to determine if the ATC is working. Hold the instrument toward a source of light and read the value directly from the specific gravity scale. This procedure is applicable to calibrate the refractometer, installed in the quality control department. Note: NIST Interagency or Internal Reports (NIST"IR") can be obtained by going to the Weight and Measures Publications page. <>>>
Using a plastic pipette, drip sample onto the prism surface. Then adjust the unit’s calibration screw so that the refractometer reads exactly zero. Hold the refractometer with the daylight plate facing upward, direct it towards the light and observe the field of view through the eyepiece. using Virkon spray and a paper towel or disinfectant wipe 3. 7.0 Installation Procedure 7 7.1 Set Up 8 8.0 Sample Reading 10 9.0 Menu Options 11 10.0 Daily Single Point Calibration 12 11.0 Multipoint Calibration 13 11.1 Calibration Oils 14 12.0 Channels 15 12.1 Channel 1 – Brix (Sucrose per ICUMSA) 15 12.2 Channels 2 – 4, … ) or https:// means you've safely connected to the .gov website. 1. First, determine whether you need a Level I or Level II Certification and use the chart below. To describe the procedure for operation and calibration of Refractometer. CALIBRATION PROCEDURE Calibration should be performed daily, before measurements are made, when the battery has been replaced, or between a long series of measurements. 3 0 obj
Repeat the experiment with different liquids and study the variation of refractive inde… 2 0 obj
Samples are measured after a simple user calibration with steam… %µµµµ
It is a good practice to verify that you have the latest version of a procedure by checking this site on a periodic basis. NOTE: OPT10 and OPT32 models are temperature stabilized within a 18°F range. <>
Level I and II certifications differ only in the amount of documentation you require. Webmaster | Contact Us | Our Other Offices, Created October 6, 2009, Updated May 31, 2019, Manufacturing Extension Partnership (MEP), New and Existing Resources for Continuing Education and Training, How to Reset Password Using the Contacts System (PDF), Quick Guide for OWM Contacts System (PDF), How to Request Publications using OWM Contacts System (PDF), How to Request Training & Transcript Using the Contacts System (PDF), The National Conference on Weights and Measures (NCWM), Section 1, Introduction (Legacy Handbook 145), Section 2, Concepts of Accuracy and Precision and Statistical Control (Not Current, Handbook 145), Section 3, Quality Assurance in a Metrology Laboratory (Superseded by, Section 7, Miscellaneous Subjects (Legacy Handbook 145), Section 8, Statistical Techniques (Updated in NISTIR 6969, 2019), Section 9, Reference Tables (Updated in NISTIR 6969, 2019). While looking into the eyepiece, turn the Calibration Screw (on the top of the refractometer) until the boundary between the upper blue field and the lower white field meet exactly on the 0.0° line. Testing wort with the refractometer It’s fairly easy to use a refractometer, it’s largely the same process as setting up for calibration. NBS Handbook 145, 1986, Handbook for the Quality Assurance of Metrological Measurements, by John K. Taylor, and Henry V. Oppermann is out of print and the majority of content has been updated and published in the publications noted below. Calibration To properly maintain the accuracy of the Mark III refractometer, calibration of the instrument on a routine basis is recommended. endobj
The right refractometer for your needs. Procedure . 2. Dispose of any glass in a sharps bin 2. Turn the refractometer scale knob to get the clear interface between the illuminated and dark regions. 3.0 RESPONSIBILITY Officer/ Executive - Quality Control 4.0 ACCOUNTABILITY Manager - Quality Control 5.0 PROCEDURE 5.1 Operations 5.1.1 Ensure that the instrument is free from dust. After this calibration procedure, your instrument is calibrated for the current ambient room temperature. Any residue on the prism surfaces will adversely affect the accuracy of the calibration. Calibration is the indispensable and documented proof that a measuring instrument works reliably in the intended value range while maintaining specified tolerances under the current operating conditions. Calibration of the refractometer Equip with refractometer Open light plate and place 2-3 drops of distilled water on main prism like the measurement of honey, the water must spreads across the prism. <>
A .gov website belongs to an official government organization in the United States. Individual sections and procedures are provided to enable periodic updates of selected content as needed and to provide downloadable copy for laboratories to review, modify, and implement as needed and as appropriate. Using a Refractometer 1. 2. A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry).The index of refraction is calculated from Snell's law while for mixtures, the index of refraction can be calculated from the composition of the material using several mixing rules such as the Gladstone–Dale relation and Lorentz–Lorenz equation MEASUREMENT PROCEDURE Verify the instrument has been calibrated before taking measurements. Two instrument test screens will be displayed briefly; an “all segment” screen followed by the percentage of remaining battery life. A lock ( LockA locked padlock 6. HB 145 was developed as a source of calibration procedures for weights and measures laboratories and covered mass, length and volume calibrations for field standards used in the commercial marketplace. %PDF-1.5
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Temperature of the sample can be varied by using the slider. Anton Paar’s Abbemat refractometer series ranges from the straightforward Abbemat 3X00 series for routine analysis to the sturdy Heavy Duty line for measurements in harsh environments. Wipe off prism surface located at the bottom of the sample well. endobj
The original Table of Contents and several Legacy sections are provided here as reference only. 5. To switch the views of the equipment, click the left and right arrows on the bottom of the simulator. Steps for Calibration 1. 4. 9). Two instrument test screens will be displayed briefly; an “all segment” screen followed by the percentage of remaining battery life. 4 0 obj
An official website of the United States government. 14 MA887 Refractometer 7. The concentration calibration of the K-Patents PR-43-A refractometer is organized in three (3) layers: the Refractometer nD calibration, chemical curve and field calibration. 3. Focus the refractometer until the scale is clearly visible. Choose the liquid sample. Note any patterns present and the overall accuracy. your calibration test kit. 2. Official websites use .gov